CD2AP Polyklonaler Antikörper

CD2AP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24122-1-AP

Synonyme

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

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  WB analysis using 24122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:400-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

24122-1-AP bindet in WB, IHC, ELISA CD2AP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD2AP fusion protein Ag21170
• Vollständiger Name: CD2-associated protein
• Berechnetes Molekulargewicht: 637 aa, 70 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC157901
• Gene symbol: Cd2ap
• Gene ID (NCBI): 12488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publikationen

Species	Application	Title
mouse	WB,IHC	Sci Rep; Disulfidptosis links the pathophysiology of ulcerative colitis and immune infiltration in colon adenocarcinoma; Authors - Chenhao Jiang
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
mouse	WB	Am J Physiol Cell Physiol; Long non-coding RNA Kcnq1ot1 promotes sC5b-9-induced podocyte pyroptosis by inhibiting miR-486a-3p and upregulating NLRP3.; Authors - Chunjian Zhang
mouse	WB	Inflammation; Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.; Authors - Soulixay Senouthai