CD68 Polyklonaler Antikörper

CD68 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(315)

Rezensionen (2)

Kat-Nr. : 28058-1-AP

Synonyme

CD68 antigen, gp110, Scard1

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  WB analysis of RAW 264.7 using 28058-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (CD68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28058-1-AP

  NR8383 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (CD68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 28058-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28058-1-AP (CD68 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (CD68 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 28058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 60 publications below
• IHC: See 124 publications below
• IF: See 153 publications below

Produktinformation

28058-1-AP bindet in WB, IHC, IF-P, ELISA CD68 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD68 fusion protein Ag27827
• Vollständiger Name: CD68 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_001291058
• Gene symbol: Cd68
• Gene ID (NCBI): 12514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publikationen

Species	Application	Title
pig	IF	Nature; Injectable ultrasonic sensor for wireless monitoring of intracranial signals; Authors - Hanchuan Tang
	WB,IF	Signal Transduct Target Ther; C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness; Authors - Jun Ma
mouse	IF	Nat Aging; Activation of AMPK by GLP-1R agonists mitigates Alzheimer-related phenotypes in transgenic mice; Authors - Yun Zhang
mouse	IHC	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
mouse	IF	ACS Nano; Inflammation and Acinar Cell Dual-Targeting Nanomedicines for Synergistic Treatment of Acute Pancreatitis via Ca2+ Homeostasis Regulation and Pancreas Autodigestion Inhibition; Authors - Yanan Wang
mouse	IF	J Pineal Res; Extracellular vesicles derived from melatonin-preconditioned mesenchymal stem cells containing USP29 repair traumatic spinal cord injury by stabilizing NRF2.; Authors - Wei Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandru (Verified Customer) (11-13-2023)

Great antibody, worked well in my experiments.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse ventricle

FH

Amelie (Verified Customer) (09-09-2019)

sections were deparaffinized and subjected to heat mediated antigen retrieval using TE buffer ph9On the pictures CD68 in red

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse adrenal gland

CD68 Antibody Immunofluorescence, validation (1:1000 dilution) in mouse adrenal gland (Cat no:28058-1-AP)