Ch-TOG Polyklonaler Antikörper

Ch-TOG Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26457-1-AP

Synonyme

CKAP5, ch TOG, Colonic and hepatic tumor overexpressed gene protein, Cytoskeleton-associated protein 5, TOGp

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  WB analysis of NCCIT using 26457-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26457-1-AP (Ch-TOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 26457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26457-1-AP (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 26457-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26457-1-AP (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26457-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (26457-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, NCCIT-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

26457-1-AP bindet in WB, IHC, IF/ICC, ELISA Ch-TOG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ch-TOG fusion protein Ag24875
• Vollständiger Name: cytoskeleton associated protein 5
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC120869
• Gene symbol: Ch-TOG
• Gene ID (NCBI): 9793
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

Publikationen

Species	Application	Title
		Cell Rep; Compartmentalized control of Cdk1 drives mitotic spindle assembly.; Authors - Angela Flavia Serpico
human	WB,IF	Clin Transl Med; Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition; Authors - Hui Luo