Chromogranin A Monoklonaler Antikörper

Chromogranin A Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3H11C11

Kat-Nr. : CL555-60135

Synonyme

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie der Validierungsdaten

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  IF Staining of PC-12 using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of SH-SY5Y using CL555-60135

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  IF Staining of Neuro-2a using CL555-60135

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite®555 Chromogranin A antibody (CL555-60135, Clone: 3H11C11 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL555-60135

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Neuro-2a using CL555-60135

  1X10^6 Neuro-2a cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human Chromogranin A (CL555-60135, Clone:3H11C11) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: PC-12-Zellen, Neuro-2a-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen, Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-60135 bindet in IF, FC (Intra) Chromogranin A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag0807
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: CHGA
• Gene ID (NCBI): 1113
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.