Collagen Type I Monoklonaler Antikörper

Collagen Type I Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

123

CloneNo.

1E9A7

Rezensionen (6)

Kat-Nr. : 67288-1-Ig

Synonyme

Alpha 1 type I collagen, COL1A1, Collagen alpha 1(I) chain, collagen, type I, alpha 1, OI4

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Galerie der Validierungsdaten

• 

  WB analysis of pig colon using 67288-1-Ig

  pig colon tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig lung using 67288-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skin using 67288-1-Ig

  pig skin tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67288-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cervical cancer using 67288-1-Ig

  human cervical cancer tissue were subjected to SDS PAGE followed by western blot with 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67288-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67288-1-Ig (Collagen Type I antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 67288-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human colon cancer using 67288-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Collagen Type I antibody (67288-1-Ig, Clone: 1E9A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Kolongewebe, Hausschwein-Hautgewebe, Hausschwein-Lungengewebe, humanes Plazenta-Gewebe, humanes Zervixkarzinomgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 95 publications below
• IHC: See 26 publications below
• IF: See 25 publications below

Produktinformation

67288-1-Ig bindet in WB, IHC, IF, ELISA Collagen Type I und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type I, alpha 1
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_000088
• Gene symbol: COL1A1
• Gene ID (NCBI): 1277
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727).

Publikationen

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	IHC	Adv Sci (Weinh); Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner; Authors - Hao Sun
	WB,IHC	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
	WB	Eur Respir J; Intracellular hydroxyproline imprinting following resolution of bleomycin-induced pulmonary fibrosis.; Authors - Shengren Song
	WB	Nat Commun; Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.; Authors - Yinan Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Niky (Verified Customer) (02-26-2024)

Arrived very quickly on ice. However, didnt work for my tissue type

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: endometrial lesion

FH

Udesh (Verified Customer) (12-22-2023)

Worked well for IF at 1:400

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: BM-MSCs

FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: human fibroblasts

FH

Ren Jie (Verified Customer) (06-22-2022)

Does not work for pepsin digested human collagen I

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Purified protein

FH

Hala (Verified Customer) (02-26-2021)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000

FH

Hala (Verified Customer) (02-23-2021)

very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000