Connexin-26 Polyklonaler Antikörper

Connexin-26 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16960-1-AP

Synonyme

GJB2, Connexin 26, CX26, DFNA3, DFNA3A

---

• 

  WB analysis using 16960-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16960-1-AP (Connexin-26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16960-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16960-1-AP (Connexin-26 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 16960-1-AP

  IP result of anti-Connexin-26 (IP:16960-1-AP, 4ug; Detection:16960-1-AP 1:3000) with PC-3 cells lysate 1200 ug.

• 

  IF Staining of PC-3 using 16960-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 16960-1-AP (Connexin-26 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, humanes Lebergewebe, Mausherzgewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

16960-1-AP bindet in WB, IF/ICC, IP, ELISA Connexin-26 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Connexin-26 fusion protein Ag10666
• Vollständiger Name: gap junction protein, beta 2, 26kDa
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC017048
• Gene symbol: Connexin-26
• Gene ID (NCBI): 2706
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin-26 (GJB2) is a beta class gap junction protein. Mutations in the gene for connexin-26 are responsible for as much as 50% of pre-lingual, recessive deafness.

Publikationen

Species	Application	Title
human	WB	Oncol Res; Microenvironment Analysis of Prognosis and Molecular Signature of Immune-Related Genes in Lung Adenocarcinoma.; Authors - Bo Ling
human	IF	Cell Commun Signal; Repression of Connexin26 hemichannel activity protects the barrier function of respiratory airway epithelial cells against LPS-induced alteration; Authors - Tina Lehrich
		Mol Med Rep; Molecular hydrogen accelerates the reversal of acute obstructive cholangitis‑induced liver dysfunction by restoring gap and tight junctions.; Authors - Zhiyang Zhu