Cyclin B1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin B1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(83)

Kat-Nr. : 28603-1-AP

Synonyme

CCNB1, CCNB, Cyclin B, G2/mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

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  WB analysis of HeLa using 28603-1-AP

  WB result of Cyclin B1 antibody (28603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin B1 transfected HeLa cells.

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  WB analysis using 28603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28603-1-AP (Cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28603-1-AP

  IP result of anti-Cyclin B1 (IP:28603-1-AP, 4ug; Detection:28603-1-AP 1:2000) with HeLa cells lysate 1800 ug.

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28603-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28603-1-AP (Cyclin B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HT-29 using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 28603-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin B1 antibody (28603-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of Ramos using 28603-1-AP

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human Cyclin B1 (28603-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, C6-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HT-29-Zellen
• Erfolgreiche Detektion in FC (Intra): Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 78 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Produktinformation

28603-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA Cyclin B1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin B1 fusion protein Ag29426
• Vollständiger Name: cyclin B1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC006510
• Gene symbol: Cyclin B1
• Gene ID (NCBI): 891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Clin Transl Med; Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3‐mediated N6‐methyladenosine modification of ICAM2 mRNA in fibroblast‐like synoviocytes; Authors - Jian Chen
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	Mol Oncol; Interaction between membranous EBP50 and myosin 9 as a favorable prognostic factor in ovarian clear cell carcinoma; Authors - Mayu Nakagawa
human	WB	Life Sci; Periplocin inhibits hepatocellular carcinoma progression and reduces the recruitment of MDSCs through AKT/NF-κB pathway; Authors - Jia-Peng Lin
rat,human	WB,IHC	Am J Physiol Lung Cell Mol Physiol; KLF5 mediates vascular remodeling via HIF-1α in hypoxic pulmonary hypertension; Authors - Xiaochen Li