Cyclin D3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cyclin D3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 26755-1-AP

Synonyme

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3

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Galerie der Validierungsdaten

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  WB analysis of C6 using 26755-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26755-1-AP

  WB result of Cyclin D3 antibody (26755-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin D3 transfected Jurkat cells.

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  WB analysis of HeLa using 26755-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26755-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26755-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 26755-1-AP

  IP Result of anti-Cyclin D3 (IP:26755-1-AP, 4ug; Detection:26755-1-AP 1:1000) with K-562 cells lysate 2800ug.

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  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 26755-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26755-1-AP (Cyclin D3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 26755-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26755-1-AP (Cyclin D3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

26755-1-AP bindet in WB, IP, IHC, IF, ELISA Cyclin D3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cyclin D3 fusion protein Ag25151
• Vollständiger Name: cyclin D3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC011616
• Gene symbol: CCND3
• Gene ID (NCBI): 896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Publikationen

Species	Application	Title
human	WB,IP	Acta Pharmacol Sin; Inhibition of USP10 induces myeloma cell apoptosis by promoting cyclin D3 degradation; Authors - Yu-Jia Xu ; KD Validated
human	WB	J Proteome Res; Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells; Authors - Alireza Delfarah
human	WB	Transl Lung Cancer Res; Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.; Authors - Zhiwei Zhang
human	IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	J Cell Mol Med; Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.; Authors - Dan Zhang
human	WB	Mol Ther Oncolytics; S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression.; Authors - Weijing He