Cytokeratin 14 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 14 Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G1E2

Kat-Nr. : CL647-60320

Synonyme

KRT14, Cytokeratin-14, Cytokeratin,Keratin, CK-14, CK14

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  IF Staining of mouse skin using CL647-60320

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using CL647-60320

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using CoraLite® Plus 647 Cytokeratin 14 antibody (CL647-60320, Clone: 2G1E2 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using CL647-60320

  1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Cytokeratin 14 (CL647-60320, Clone:2G1E2) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushautgewebe
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60320 bindet in IF-P, FC (Intra) Cytokeratin 14 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 14 fusion protein Ag17559
• Vollständiger Name: keratin 14
• Berechnetes Molekulargewicht: 472 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC002690
• Gene symbol: Cytokeratin 14
• Gene ID (NCBI): 3861
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.