Cytokeratin 18 Monoklonaler Antikörper
Cytokeratin 18 Monoklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1G11C4
Kat-Nr. : CL647-66187
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
| Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Durchflusszytometrie (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL647-66187 bindet in IF/ICC, FC (Intra) Cytokeratin 18 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Cytokeratin 18 fusion protein Ag1260 |
| Vollständiger Name | keratin 18 |
| Berechnetes Molekulargewicht | 48 kDa |
| Beobachtetes Molekulargewicht | 48 kDa |
| GenBank-Zugangsnummer | BC000180 |
| Gene symbol | Cytokeratin 18 |
| Gene ID (NCBI) | 3875 |
| Konjugation | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL Plus 647 Cytokeratin 18 antibody CL647-66187 | Protokoll herunterladen |
| FC protocol for CL Plus 647 Cytokeratin 18 antibody CL647-66187 | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |

