Cytokeratin 18 Monoklonaler Antikörper
Cytokeratin 18 Monoklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (1)
Anwendung
IF/ICC, FC (Intra)
Konjugation
FITC Fluorescent Dye
CloneNo.
1G11C4
Kat-Nr. : FITC-66187
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Erfolgreiche Detektion in FC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
IF | See 3 publications below |
FC | See 1 publications below |
Produktinformation
FITC-66187 bindet in IF/ICC, FC (Intra) Cytokeratin 18 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Rind |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Cytokeratin 18 fusion protein Ag1260 |
Vollständiger Name | keratin 18 |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 48 kDa |
GenBank-Zugangsnummer | BC000180 |
Gene symbol | Cytokeratin 18 |
Gene ID (NCBI) | 3875 |
Konjugation | FITC Fluorescent Dye |
Excitation/Emission maxima wavelengths | 498 nm / 526 nm |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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IF protocol for FITC Cytokeratin 18 antibody FITC-66187 | Protokoll herunterladen |
FC protocol for FITC Cytokeratin 18 antibody FITC-66187 | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Dairy Sci Diacylglycerol O-acyltransferase (DGAT) isoforms play a role in peridroplet mitochondrial fatty acid metabolism in bovine liver | ||
Exp Ther Med Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer | ||
J Dairy Sci Caveolin 1 in bovine liver is associated with fatty acid-induced lipid accumulation and the ER unfolded protein response: role in fatty liver development |