Cytokeratin 18 Monoklonaler Antikörper

Cytokeratin 18 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

IF/ICC, FC (Intra)

Konjugation

FITC Fluorescent Dye

Publikationen(4)

CloneNo.

1G11C4

Kat-Nr. : FITC-66187

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18



Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 3 publications below
FCSee 1 publications below

Produktinformation

FITC-66187 bindet in IF/ICC, FC (Intra) Cytokeratin 18 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Rind
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Cytokeratin 18 fusion protein Ag1260
Vollständiger Name keratin 18
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 48 kDa
GenBank-ZugangsnummerBC000180
Gene symbol Cytokeratin 18
Gene ID (NCBI) 3875
Konjugation FITC Fluorescent Dye
Excitation/Emission maxima wavelengths498 nm / 526 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for FITC Cytokeratin 18 antibody FITC-66187Protokoll herunterladen
FC protocol for FITC Cytokeratin 18 antibody FITC-66187Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
bovineFC

J Dairy Sci

Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver

Authors - Wei Yang
bovineIF

J Dairy Sci

Diacylglycerol O-acyltransferase (DGAT) isoforms play a role in peridroplet mitochondrial fatty acid metabolism in bovine liver

Authors - Shuang Wang
humanIF

Exp Ther Med

Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer

Authors - Jianping Jiang
bovineIF

J Dairy Sci

Caveolin 1 in bovine liver is associated with fatty acid-induced lipid accumulation and the ER unfolded protein response: role in fatty liver development

Authors - Ming Li