Cytokeratin 19 Polyklonaler Antikörper

Cytokeratin 19 Polyklonal Antikörper für IF/ICC, IF-Fro, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-Fro, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-10712

Synonyme

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

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  IF Staining of mouse small intestine using CL488-10712

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-10712

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HeLa using CL488-10712

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HeLa using CL488-10712

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-10712

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Cytokeratin 19 antibody (CL488-10712) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-10712

  Adult mouse small intestine stained for Cytokeratin 19 (green, CL488-10712) and BMI1 (red, 10832-1-AP). BMI1 stains intestinal stem cells located at the base of the villi. As these stem cells differentiate into intestinal epithelial cells, they acquire Cytokeratin 19 expression. Cytokeratin 19 was conjugated to Coralite 488. Samples were fixed in 4% PFA, embedded in paraffin, and imaged on a confocal microscope. Image credit: @Immunofluorescence on Instagram.

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  FC experiment of MCF-7 using CL488-10712

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 19 (CL488-10712) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-10712 bindet in IF/ICC, IF-Fro, FC (Intra) Cytokeratin 19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC007628
• Gene symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.