Cytokeratin 19 Monoklonaler Antikörper

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• KD/KO Validated

Cytokeratin 19 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3G1E4

Kat-Nr. : CL647-60187

Synonyme

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Galerie der Validierungsdaten

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  IF Staining of human breast cancer using CL647-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL647-60187

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 Cytokeratin 19 antibody (CL647-60187, Clone: 3G1E4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-60187

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cytokeratin 19 (CL647-60187, Clone:3G1E4) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60187 bindet in IF, FC (Intra) Cytokeratin 19 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 19 fusion protein Ag7407
• Vollständiger Name: keratin 19
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC002539
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C