Cytokeratin 20 Monoklonaler Antikörper

Cytokeratin 20 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

4D10A4

Kat-Nr. : CL488-60183

Synonyme

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

---

• 

  IF Staining of human colon using CL488-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200, CoraLite®594 CD68 antibody (CL594-25747, red).

• 

  IF Staining of human colon using CL488-60183

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200, CoraLite®594 CD68 antibody (CL594-25747, red).

• 

  IF Staining of HT-29 using CL488-60183

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using CoraLite® Plus 488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4 ) at dilution of 1:200.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-60183 bindet in IF/ICC, IF-P Cytokeratin 20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 20 fusion protein Ag11336
• Vollständiger Name: keratin 20
• Berechnetes Molekulargewicht: 424 aa, 48 kDa
• GenBank-Zugangsnummer: BC031559
• Gene symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.

Publikationen

Species	Application	Title
mouse	IF	Nat Neurosci; Emergence of a brainstem somatosensory tonotopic map for substrate vibration; Authors - Kuo-Sheng Lee