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Cytokeratin 7 Polyklonaler Antikörper

Cytokeratin 7 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

Kat-Nr. : CL555-22208

Synonyme

CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated Cytokeratin 7 Polyclonal a (CL555-22208)

    IF Staining of HeLa using CL555-22208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-22208) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated Cytokeratin 7 Polyclonal a (CL555-22208)

    IF Staining of HeLa using CL555-22208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Cytokeratin 7 antibody (CL555-22208) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Cytokeratin 7 Polyclonal a (CL555-22208)

    FC experiment of HeLa using CL555-22208

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Cytokeratin 7 (CL555-22208) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-22208 bindet in IF, FC (Intra) Cytokeratin 7 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cytokeratin 7 fusion protein Ag17528
Vollständiger Name keratin 7
Berechnetes Molekulargewicht 469 aa, 51 kDa
GenBank-ZugangsnummerBC002700
Gene symbol KRT7
Gene ID (NCBI) 3855
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 Cytokeratin 7 antibody CL555-22208Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen