CCAR2 Polyklonaler Antikörper

CCAR2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 22638-1-AP

Synonyme

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

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  WB analysis using 22638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22638-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22638-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22638-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22638-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22638-1-AP

  IP result of anti-CCAR2 (IP:22638-1-AP, 4ug; Detection:22638-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 22638-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA1967 antibody 22638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:8000-1:32000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

22638-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA CCAR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCAR2 fusion protein Ag18374
• Vollständiger Name: KIAA1967
• Berechnetes Molekulargewicht: 923 aa, 103 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC018269
• Gene symbol: CCAR2
• Gene ID (NCBI): 57805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	CoIP,RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
mouse	WB, IF	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human	WB,IHC,IP	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu
mouse	IF	Br J Pharmacol; AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease; Authors - Fengjiao Sun
human	WB	Redox Biol; SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3; Authors - Xi Chen
rat	WB	Free Radic Biol Med; CircMETTL9 targets CCAR2 to induce neuronal oxidative stress and apoptosis via mitochondria-mediated pathways following traumatic brain injury; Authors - Hao Huang