DCBLD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DCBLD2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13168-1-AP

Synonyme

ESDN, Endothelial and smooth muscle cell-derived neuropilin-like protein, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, CLCP1

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  WB analysis of human liver using 13168-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13168-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13168-1-AP (DCBLD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 13168-1-AP

  IP result of anti-DCBLD2 (IP:13168-1-AP, 4ug; Detection:13168-1-AP 1:1000) with U2OS cells lysate 440 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 13168-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13168-1-AP (DCBLD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 13168-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13168-1-AP (DCBLD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MKN-45-Zellen, humanes Hirngewebe, humanes Lebergewebe, SW 1990-Zellen, U2OS-Zellen
• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

13168-1-AP bindet in WB, IHC, IP, ELISA DCBLD2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCBLD2 fusion protein Ag3821
• Vollständiger Name: discoidin, CUB and LCCL domain containing 2
• Berechnetes Molekulargewicht: 775 aa, 89 kDa
• Beobachtetes Molekulargewicht: 93 kDa, 127 kDa
• GenBank-Zugangsnummer: BC029658
• Gene symbol: DCBLD2
• Gene ID (NCBI): 131566
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

Publikationen

Species	Application	Title
human	WB	Environ Toxicol; Comprehensive analyses and experimental verification of NETs and an EMT gene signature for prognostic prediction, immunotherapy, and chemotherapy in pancreatic adenocarcinoma; Authors - Jing Zhang ; KD Validated
human	WB,IHC	Am J Cancer Res; In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer; Authors - Qiankun Zhu
mouse	WB	Aging (Albany NY); Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin; Authors - Yuqi Xin