DCLRE1B Polyklonaler Antikörper

DCLRE1B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 13203-1-AP

Synonyme

5 exonuclease Apollo, 5' exonuclease Apollo, APOLLO, Beta-lactamase DCLRE1B, DNA cross-link repair 1B protein

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  WB analysis of Jurkat using 13203-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13203-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13203-1-AP (DCLRE1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13203-1-AP bindet in WB, ELISA DCLRE1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCLRE1B fusion protein Ag3945
• Vollständiger Name: DNA cross-link repair 1B (PSO2 homolog, S. cerevisiae)
• Berechnetes Molekulargewicht: 532 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC029687
• Gene symbol: DCLRE1B
• Gene ID (NCBI): 64858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interstrand cross-links (ICLs) is an extremely cytotoxic form of DNA damage that covalently tethers the two strands of the double helix, which inhibits fundamental nuclear functions, including transcription and DNA replication [PMID:21701511]. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links [PMID:10848582]. Furthermore, DCLRE1B was identified as a TRF2-interacting protein and, has a role in telomere maintenance in association with the Shelterin complex, preventing non-homologous end-joining-mediated chromosome fusions protecting leading strand telomeres [PMID:20619712].

Publikationen

Species	Application	Title
human	WB	Nat Struct Mol Biol; Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development; Authors - Nazareno Bona
human	WB	Sci Rep; DCLRE1B as a novel prognostic biomarker associated with immune infiltration: a pancancer analysis; Authors - Mi Zou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (02-24-2025)

Antibody does not specifically recognize DCLRE1B. A band can still be detected in DCLRE1B knockdown cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE1