DCT Polyklonaler Antikörper

DCT Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13095-1-AP

Synonyme

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

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Galerie der Validierungsdaten

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 13095-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 13095-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13095-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13095-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13095-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13095-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13095-1-AP (DCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 13095-1-AP

  IP Result of anti-DCT (IP:13095-1-AP, 4ug; Detection:13095-1-AP 1:500) with HL-60 cells lysate 4000ug.

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 13095-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13095-1-AP (DCT antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 13095-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (13095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, A375-Zellen, A431-Zellen, COS-7-Zellen, MCF-7-Zellen, Rattenhautgewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

13095-1-AP bindet in WB, IP, IHC, IF, ELISA DCT und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCT fusion protein Ag3714
• Vollständiger Name: dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
• Berechnetes Molekulargewicht: 519 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC028311
• Gene symbol: DCT
• Gene ID (NCBI): 1638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Publikationen

Species	Application	Title
mouse	IF	Curr Biol; Neuropsin (OPN5) Mediates Local Light-Dependent Induction of Circadian Clock Genes and Circadian Photoentrainment in Exposed Murine Skin.; Authors - Ethan D Buhr
human	IHC	Hum Mol Genet; Transcriptome analysis and molecular signature of human retinal pigment epithelium.; Authors - Strunnikova N V NV
human	WB	J Invest Dermatol; miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.; Authors - Chonglin Luo
human	WB	J Cell Mol Med; Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes; Authors - Yihui Xie
human	WB	Sci Rep; Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.; Authors - Sabrina Fehrenbach
mouse	WB	Pharmaceutics; Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion.; Authors - Xudong Tang