DCTD Polyklonaler Antikörper

DCTD Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-16784

Synonyme

EC:3.5.4.12, Deoxycytidylate deaminase, dCMP deaminase

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  IF Staining of HepG2 using CL488-16784

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DCTD antibody (CL488-16784) at dilution of 1:200.

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  FC experiment of HepG2 using CL488-16784

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DCTD Polyclonal Antibody (CL488-16784)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-16784 bindet in IF/ICC, FC (Intra) DCTD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DCTD fusion protein Ag10216
• Vollständiger Name: dCMP deaminase
• Berechnetes Molekulargewicht: 178 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC001286
• Gene symbol: DCTD
• Gene ID (NCBI): 1635
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DCTD(Deoxycytidylate deaminase) is also named as dCMP deaminase and belongs to the cytidine and deoxycytidylate deaminase family. It catalyzes the deamination of dCMP to dUMP, thus providing the nucleotide substrate for thymidylate synthase. Control of deaminase activity at this juncture in deoxyribonucleotide metabolism is determined by the ratio of dCTP to dTTP in the cell, since the enzyme is allosterically activated by dCTP and inhibited by dTTP(PMID:7685356). It has 2 isoforms produced by alternative splicing and can exsit as a dimer(pMID:8798492).