DDX17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX17 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen(21)

Rezensionen (2)

Kat-Nr. : 19910-1-AP

Synonyme

DDX 17, DEAD box protein 17, DEAD box protein p72, DEAD box protein p82, EC:3.6.4.13

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19910-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19910-1-AP

  IP result of anti-DDX17 (IP:19910-1-AP, 4ug; Detection:19910-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 10x lens).

• 

  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 10x lens).

• 

  IF Staining of HEK-293 using 19910-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDX17 antibody (19910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HEK-293T-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 4 publications below

Produktinformation

19910-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, PLA DDX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX17 fusion protein Ag13723
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• Berechnetes Molekulargewicht: 729 aa, 80 kDa
• Beobachtetes Molekulargewicht: 72-80 kDa
• GenBank-Zugangsnummer: BC000595
• Gene symbol: DDX17
• Gene ID (NCBI): 10521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. This antibody could detect the 72 kDa isoform and 80 kDa isoform of DDX17.

Publikationen

Species	Application	Title
human	CoIP,chIP	Nat Commun; Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1.; Authors - Lamya Ben Ameur
human	IP	Nucleic Acids Res; The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.; Authors - Marie-Pierre Lambert
human	WB,IP	Nucleic Acids Res; RNA helicase-dependent gene looping impacts messenger RNA processing; Authors - Sophie Terrone ; KD Validated
mouse,human	WB,IHC	Clin Transl Med; DEAD-Box Helicase 17 exacerbates non-alcoholic steatohepatitis via transcriptional repression of cyp2c29, inducing hepatic lipid metabolism disorder and eliciting the activation of M1 macrophages; Authors - Deng Ning
human	WB	PLoS Pathog; Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.; Authors - Hélène Chabrolles
	WB	Front Immunol; HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.; Authors - Mei-Ling Dong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Great antibody to detect DDX17 by WB (Overnight incubation at 4°C, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

N (Verified Customer) (01-28-2023)

Perfect to detect double bands of DDX17.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

DDX17 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:19910-1-AP)