DDX60 Rekombinanter Antikörper

DDX60 Rekombinant Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230293G10

Kat-Nr. : 82969-4-RR

Synonyme

230293G10, DEAD box protein 60, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX60

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  IHC staining of human stomach using 82969-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82969-4-RR (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach using 82969-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach tissue using DDX60 antibody (82969-4-RR, Clone: 230293G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 82969-4-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human DDX60 (82969-4-RR, Clone:230293G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Magengewebe
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82969-4-RR bindet in IHC, IF-P, FC (Intra), ELISA DDX60 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DDX60 fusion protein Ag34406
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• Berechnetes Molekulargewicht: 1712 aa, 198 kDa
• Beobachtetes Molekulargewicht: 170-200 kDa
• GenBank-Zugangsnummer: BC038115
• Gene symbol: DDX60
• Gene ID (NCBI): 55601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.