DEPDC6/Deptor Polyklonaler Antikörper

DEPDC6/Deptor Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 20985-1-AP

Synonyme

DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 20985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20985-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20985-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 20985-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20985-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DEPDC6/Deptor antibody (20985-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A2780-Zellen, COLO 320-Zellen, HEK-293-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

20985-1-AP bindet in WB, IHC, IF, ELISA DEPDC6/Deptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DEP domain containing 6
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_022783
• Gene symbol: DEPDC6
• Gene ID (NCBI): 64798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

Publikationen

Species	Application	Title
human	WB,IHC	Sci Transl Med; The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.; Authors - Fabiana Csukasi
human	WB	Autophagy; TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.; Authors - Yue Zhai
human	WB	Cell Death Differ; NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.; Authors - Hao Li
mouse	WB	Oncotarget; Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.; Authors - Mengmeng Cheng
mouse	IF	Stem Cell Res Ther; DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.; Authors - Si Chen
mouse	WB	Gene; p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.; Authors - Xiaoling Yu