DHODH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DHODH Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(93)

Rezensionen (2)

Kat-Nr. : 14877-1-AP

Synonyme

PYRD, EC:1.3.5.2, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone), mitochondrial, dihydroorotate dehydrogenase

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  WB analysis using 14877-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14877-1-AP (DHODH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 14877-1-AP

  IP result of anti-DHODH (IP:14877-1-AP, 4ug; Detection:14877-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14877-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14877-1-AP (DHODH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 14877-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DHODH antibody (14877-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293T using 14877-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.2 ug Anti-Human DHODH (14877-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, MCF-7-Zellen, Mausherzgewebe, Maus-Eierstockgewebe, Mausmilzgewebe, Rattenmilzgewebe, SKOV-3-Zellen
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in FC (Intra): HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 86 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

14877-1-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA DHODH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DHODH fusion protein Ag6649
• Vollständiger Name: dihydroorotate dehydrogenase
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC065245
• Gene symbol: DHODH
• Gene ID (NCBI): 1723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publikationen

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB	Nature; DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.; Authors - Chao Mao ; KO Validated
human,mouse	WB	Science; Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1.; Authors - Issam Ben-Sahra
human,mouse	WB	Cancer Discov; PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition.; Authors - Deepti Mathur
mouse	IHC	Cancer Cell; Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma; Authors - William D Gwynne ; KO Validated
mouse	WB	Cell Metab; Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.; Authors - Christopher J Halbrook ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded kidneys were stained for Dhodh (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

DHODH Antibody Immunofluorescence validation (1:200 dilution) in Mouse kidney (Cat no:14877-1-AP)

FH

Daniel (Verified Customer) (04-16-2019)

Highly specific detection of both mouse and human DHODH.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human RCC4 cells (lane1), Mouse kidney (lane2)

DHODH Antibody Western Blot, validation (1:1000 dilution) in Human RCC4 cells (lane1), Mouse kidney (lane2) (Cat no:14877-1-AP)