DLX1 Polyklonaler Antikörper

DLX1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13046-1-AP

Synonyme

Homeobox protein DLX-1

---

• 

  WB analysis of human brain using 13046-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13046-1-AP (DLX1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13046-1-AP (DLX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13046-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13046-1-AP (DLX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13046-1-AP (DLX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13046-1-AP (DLX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, A375-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13046-1-AP bindet in WB, IHC, ELISA DLX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DLX1 fusion protein Ag3586
• Vollständiger Name: distal-less homeobox 1
• Berechnetes Molekulargewicht: 255 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC036189
• Gene symbol: DLX1
• Gene ID (NCBI): 1745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Distal-less homeobox 1(DLX1) is a member of the DLX family of homeobox transcription factors, which is essential for the production of forebrain GABAergic interneurions during embryonic development. The DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6) is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX1 possible has a role in craniofacial patterning and morphogenesis and involves in the early development of diencephalic subdivisions.

Publikationen

Species	Application	Title
human	WB	Cancer Manag Res; miR-489-3p Inhibits Prostate Cancer Progression by Targeting DLX1.; Authors - Peide Bai
mouse	WB	Cell Death Differ; Cullin 4B-RING E3 ligase negatively regulates the immunosuppressive capacity of mesenchymal stem cells by suppressing iNOS; Authors - Ruiqi Yu