Dystrophin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Dystrophin Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 12715-1-AP

Synonyme

BMD, CMD3B, DMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, dystrophin

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Galerie der Validierungsdaten

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  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12715-1-AP

  WB result of Dystrophin antibody (12715-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dystrophin transfected HepG2 cells.

• 

  WB analysis of HepG2 using 12715-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12715-1-AP (Dystrophin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12715-1-AP

  IP result of anti-Dystrophin(IP:12715-1-AP, 4ug; Detection:12715-1-AP 1:20000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of human skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12715-1-AP (Dystrophin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 12715-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12715-1-AP (Dystrophin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 12715-1-AP (Dystrophin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle tissue using 12715-1-AP

  IF result of anti-DMD (12715-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

• 

  IF Staining of rat heart using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Connexin 43 antibody (26980-1-AP, green).

• 

  IF Staining of mouse skeletal muscle using 12715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Dystrophin antibody (12715-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maus-Skelettmuskelgewebe, Rattenherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 12 publications below
• IP: See 2 publications below

Produktinformation

12715-1-AP bindet in WB, IP, IHC, IF, ELISA Dystrophin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Dystrophin fusion protein Ag3408
• Vollständiger Name: dystrophin
• Berechnetes Molekulargewicht: 3685 aa, 427 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 430 kDa
• GenBank-Zugangsnummer: BC028720
• Gene symbol: DMD
• Gene ID (NCBI): 1756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Publikationen

Species	Application	Title
mouse	WB	Nat Biomed Eng; Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.; Authors - Ningning Shi
mouse	WB,IF	Sci Adv; Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders; Authors - Jihad El Andari
mouse	WB,IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
mouse	IF	Oxid Med Cell Longev; Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.; Authors - Wei Zou
rat	IHC	Oxid Med Cell Longev; Knockdown of Salusin-β Improves Cardiovascular Function in Myocardial Infarction-Induced Chronic Heart Failure Rats.; Authors - Yu Xu
mouse	IF	Nanomedicine; Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination; Authors - JingWen Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lauren (Verified Customer) (08-10-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: stem cell-derived myotubes

Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)

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