DNASE1L3 Polyklonaler Antikörper

DNASE1L3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11330-1-AP

Synonyme

Deoxyribonuclease gamma, deoxyribonuclease I like 3, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein DHP2, DNase I like 3, DNASE1L3, Liver and spleen DNase, LS DNase, LSD

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Galerie der Validierungsdaten

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  WB analysis of Raji using 11330-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11330-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11330-1-AP (DNASE1L3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11330-1-AP bindet in WB, ELISA DNASE1L3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNASE1L3 fusion protein Ag1873
• Vollständiger Name: deoxyribonuclease I-like 3
• Berechnetes Molekulargewicht: 305 aa, 36 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC015831
• Gene symbol: DNASE1L3
• Gene ID (NCBI): 1776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+ dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences [PMID:15796714]. It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3'-OH/5'-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin [PMID:9665719]. In addition, it has been suggested to be involved in apoptotic DNA fragmentation of thymocytes neuronal PC12 cells, C2C12 myoblasts and of cell lines expressing the recombinant nuclease [PMID: 7957253, 9307016]