DOC2B Polyklonaler Antikörper

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• KD/KO Validated

DOC2B Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20574-1-AP

Synonyme

Doc2 beta, DOC2B, DOC2BL, double C2 like domains, beta

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20574-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20574-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20574-1-AP (DOC2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20574-1-AP

  Immunofluorescent analysis of HepG2 cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20574-1-AP

  Immunofluorescent analysis of Hela cells, using DOC2B antibody 20574-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

20574-1-AP bindet in WB, IF, CoIP, ELISA DOC2B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: double C2-like domains, beta
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_003585
• Gene symbol: DOC2B
• Gene ID (NCBI): 8447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.

Publikationen

Species	Application	Title
mouse	WB,IF	Diabetes; Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function.; Authors - Arianne Aslamy ; KO Validated
human	WB	Cell Biol Toxicol; Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence.; Authors - Samatha Bhat ; KD Validated
human	WB,CoIP	Pharmacol Res; DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer.; Authors - Divya Adiga ; KD Validated
human	WB	J Biol Chem; DNA Promoter Methylation Dependent Transcription of Double C2 Like Domain Beta (DOC2B) Gene Regulates Tumor Growth in Human Cervical Cancer.; Authors - Shama Prasada Kabekkodu
human	WB,IF	Free Radic Biol Med; Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function; Authors - Divya Adiga
mouse,rat	WB	Int J Mol Sci; A Novel Role for DOC2B in Ameliorating Palmitate-Induced Glucose Uptake Dysfunction in Skeletal Muscle Cells via a Mechanism Involving β-AR Agonism and Cofilin; Authors - Jinhee Hwang