DOCK180 Polyklonaler Antikörper

DOCK180 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 23421-1-AP

Synonyme

ced5, dedicator of cytokinesis 1, DOCK1, DOCK180

---

Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 23421-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 23421-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 23421-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23421-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23421-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23421-1-AP

  IP Result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug.

• 

  IHC staining of human colon using 23421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.

• 

  IHC staining of human colon using 23421-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50.

• 

  IF Staining of HeLa using 23421-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, LNCaP-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

23421-1-AP bindet in WB, IP, IHC, IF, ELISA DOCK180 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DOCK180 fusion protein Ag18069
• Vollständiger Name: dedicator of cytokinesis 1
• Berechnetes Molekulargewicht: 1865 aa, 215 kDa
• Beobachtetes Molekulargewicht: 215 kDa
• GenBank-Zugangsnummer: BC146857
• Gene symbol: DOCK1
• Gene ID (NCBI): 1793
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Diabetes; Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing.; Authors - Hua Qu
mouse	WB	Cell Death Dis; Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways.; Authors - Xujuan Sun
zebrafish	WB	bioRxiv; Dock1 acts cell-autonomously in Schwann cells to regulate the development, maintenance, and repair of peripheral myelin; Authors - Ryan A Doan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

k. (Verified Customer) (10-26-2023)

I used it for WB with 5% BSA+TBST at 4°C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1;500
• Cell Tissue Type: Human Liver cells