DRD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DRD2 Polyklonal Antikörper für WB, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Rezensionen (2)

Kat-Nr. : 55084-1-AP

Synonyme

D(2) dopamine receptor, D2 dopamine receptor, D2DR, D2R, Dopamine D2 receptor

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  WB analysis using 55084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 55084-1-AP

  IP result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 55084-1-AP (DRD2 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 13 publications below

Produktinformation

55084-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA DRD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dopamine receptor D2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_016574
• Gene symbol: DRD2
• Gene ID (NCBI): 1813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Publikationen

Species	Application	Title
human	WB	Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
rat		Nat Commun; Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis; Authors - Nathan Bénac
mouse	WB	Mol Psychiatry; ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.; Authors - Ying Huang
rat	WB	J Affect Disord; Pramipexole improves depression-like behavior in diabetes mellitus with depression rats by inhibiting NLRP3 inflammasome-mediated neuroinflammation and preventing impaired neuroplasticity; Authors - Ping Li
rat	WB	Int J Mol Sci; Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration; Authors - Subramaniam Jayanthi
mouse	WB	J Neuroimmune Pharmacol; 5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota; Authors - Chun Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

ARUNASALAM (Verified Customer) (05-29-2025)

This antibody worked for western blotting.

• Applications: Western Blot
• Cell Tissue Type: Human Cancer Cell Lines

FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat brain tissue