DRD2 Polyklonaler Antikörper

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• KD/KO Validated

DRD2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

31

Rezensionen (1)

Kat-Nr. : 55084-1-AP

Synonyme

D(2) dopamine receptor, D2DR, D2R, Dopamine D2 receptor, dopamine receptor D2, DRD2

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Galerie der Validierungsdaten

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  WB analysis using 55084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55084-1-AP (DRD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 55084-1-AP

  IP Result of anti-DRD2 (IP:55084-1-AP, 4ug; Detection:55084-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55084-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DRD2 antibody (55084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 5 publications below
• IF: See 8 publications below

Produktinformation

55084-1-AP bindet in WB, IP, IHC, IF, ELISA DRD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dopamine receptor D2
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: NM_016574
• Gene symbol: DRD2
• Gene ID (NCBI): 1813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.

Publikationen

Species	Application	Title
human		Cell; PMID: 25594175; Authors - Yiqing Yan ; KD Validated
mouse		Mol Psychiatry; ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors.; Authors - Ying Huang
rat		Int J Mol Sci; Biochemical Neuroadaptations in the Rat Striatal Dopaminergic System after Prolonged Exposure to Methamphetamine Self-Administration; Authors - Subramaniam Jayanthi
mouse		J Neuroimmune Pharmacol; 5-HT4 Receptor is Protective for MPTP-induced Parkinson's Disease Mice Via Altering Gastrointestinal Motility or Gut Microbiota; Authors - Chun Cui
human	IHC	Cancers (Basel); Targeting Dopamine Receptor D2 by Imipridone Suppresses Uterine Serous Cancer Malignant Phenotype.; Authors - Wen Hu
mouse		Cancers (Basel); Targeting Cancer Stem Cells with Differentiation Agents as an Alternative to Genotoxic Chemotherapy for the Treatment of Malignant Testicular Germ Cell Tumors.; Authors - Amanda R Loehr

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ping (Verified Customer) (06-04-2019)

This antibody is good for both immunoprecipitation and western blot.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat brain tissue

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