DYKDDDDK tag Rekombinanter Antikörper
DYKDDDDK tag Rekombinant Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
rekombinanten Protein
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240568A11
Kat-Nr. : CL488-80801-2
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | Transfizierte HEK-293-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HEK-293T transfected with TDP-43-flag |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL488-80801-2 bindet in IF/ICC, FC (Intra) DYKDDDDK tag und zeigt Reaktivität mit rekombinanten Protein
| Getestete Reaktivität | rekombinanten Protein |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Rekombinant |
| Typ | Antikörper |
| Immunogen | DYKDDDDK tag fusion protein Ag2329 |
| Vollständiger Name | DYKDDDDK tag |
| Gene symbol | |
| Gene ID (NCBI) | 8 |
| Konjugation | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80801-2 | Protokoll herunterladen |
| FC protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80801-2 | Download protocol |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH marjolein (Verified Customer) (07-25-2025) | works as it should be
|
FH Fabian (Verified Customer) (04-25-2025) | I compared the antibody in question with the older Antibody (Prod.-No. 80810-1-RR) which is no longer available. In my setting, I tested sample from a purified protein complex which I am working on. I noticed that the older antibody does detect unspecific bands, while the newer antibody in question seems to be more specific. The intensities of the detected signal was comparable and similar. Please find my results attached.
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