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Decorin Monoklonaler Antikörper

Decorin Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A4E4

Kat-Nr. : CL488-66847

Synonyme

DCN, PG40, PG S2, DSPG2, CSCD

Filter:
  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using CoraLite® Plus 488-conjugated Decorin Monoclonal a (CL488-66847)

    IF Staining of BxPC-3 using CL488-66847

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 Decorin antibody (CL488-66847, Clone: 3A4E4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of BxPC-3 cells using CoraLite® Plus 488-conjugated Decorin Monoclonal a (CL488-66847)

    FC experiment of BxPC-3 using CL488-66847

    1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Decorin Monoclonal antibody (CL488-66847, Clone:3A4E4)(red), or 0.4 ug CoraLite® Plus 488-conjugated Mouse IgG1 isotype control Mouse McAb (CL488-66360, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCBxPC-3-Zellen
Erfolgreiche Detektion in FC (Intra)BxPC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66847 bindet in IF/ICC, FC (Intra) Decorin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Decorin fusion protein Ag6275
Vollständiger Name decorin
Berechnetes Molekulargewicht 359 aa, 40 kDa
Beobachtetes Molekulargewicht 40 kDa, 100 kDa
GenBank-ZugangsnummerBC005322
Gene symbol Decorin
Gene ID (NCBI) 1634
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 Decorin antibody CL488-66847Protokoll herunterladen
FC protocol for CL Plus 488 Decorin antibody CL488-66847Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen