Dystroglycan Monoklonaler Antikörper

Dystroglycan Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B1G12

Kat-Nr. : CL488-66735

Synonyme

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan

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Galerie der Validierungsdaten

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  IF Staining of mouse heart using CL488-66735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Dystroglycan antibody (CL488-66735, Clone: 2B1G12 ) at dilution of 1:200.

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  IF Staining of mouse heart using CL488-66735

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Dystroglycan antibody (CL488-66735, Clone: 2B1G12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-66735

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Dystroglycan (CL488-66735, Clone:2B1G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mausherzgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66735 bindet in IF, FC (Intra) Dystroglycan und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dystroglycan fusion protein Ag27222
• Vollständiger Name: dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 43 kDa, 30 kDa
• GenBank-Zugangsnummer: BC012740
• Gene symbol: DAG1
• Gene ID (NCBI): 1605
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).