E2F1 Polyklonaler Antikörper

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• KD/KO Validated

E2F1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(54)

Kat-Nr. : 12171-1-AP

Synonyme

Retinoblastoma-associated protein 1, RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1

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  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12171-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12171-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12171-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12171-1-AP (E2F1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12171-1-AP (E2F1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 48 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12171-1-AP bindet in WB, IHC, IF, IP, ELISA E2F1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E2F1 fusion protein Ag2813
• Vollständiger Name: E2F transcription factor 1
• Berechnetes Molekulargewicht: 437 aa, 47 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC050369
• Gene symbol: E2F1
• Gene ID (NCBI): 1869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein. The sumoylated E2F1 is bout 55 kDa.

Publikationen

Species	Application	Title
mouse	IHC	J Clin Invest; TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.; Authors - Zheng Hu
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	Cancer Lett; The oncoprotein HBXIP up-regulates SCG3 through modulating E2F1 and miR-509-3p in hepatoma cells.; Authors - Yue Wang ; KD Validated
mouse	IHC	Int J Nanomedicine; E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester); Authors - Jinfeng Xiong
human	IF	Mol Ther; Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.; Authors - Da Zhu
human	WB	Cell Death Dis; SEC61G promotes breast cancer development and metastasis via modulating glycolysis and is transcriptionally regulated by E2F1.; Authors - Jingjing Ma ; KD Validated