E2F4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

E2F4 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10923-1-AP

Synonyme

E2F 4, E2F4, Transcription factor E2F4

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10923-1-AP

  WB result of E2F4 antibody (10923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F4 transfected HeLa cells.

• 

  WB analysis of A431 using 10923-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10923-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10923-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10923-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10923-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10923-1-AP

  IP Result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human lymphoma using 10923-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10923-1-AP (E2F4 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

10923-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA E2F4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: E2F4 fusion protein Ag1365
• Vollständiger Name: E2F transcription factor 4, p107/p130-binding
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC033180
• Gene symbol: E2F4
• Gene ID (NCBI): 1874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Publikationen

Species	Application	Title
mouse	WB,ChIP	Protein Cell; Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.; Authors - Yuting Wang
human	WB	Oncogene; Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.; Authors - Wei Huang
mouse	WB,IHC	J Cell Physiol; Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.; Authors - Xiwen Zhang
human	WB	PLoS One; Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.; Authors - Valle Blanca L BL
human	WB	Cell Death Dis; Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.; Authors - Yinghui Wang ; KD Validated
human	WB	Cancers (Basel); Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression.; Authors - Margie N Sutton