ECHS1 Polyklonaler Antikörper

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• KD/KO Validated

ECHS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(31)

Kat-Nr. : 11305-1-AP

Synonyme

EC:4.2.1.17, ECHM, Enoyl-CoA hydratase 1, Enoyl-CoA hydratase, mitochondrial, mECH

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  WB analysis of MCF-7 using 11305-1-AP

  WB result of ECHS1 antibody (11305-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.

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  WB analysis using 11305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11305-1-AP

  WB result of ECHS1 antibody (11305-1-AP, 1:1200) with si-Control and si-ECHS1 transfected HepG2 cells. .

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  WB analysis of HeLa using 11305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11305-1-AP (ECHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11305-1-AP

  IP result of anti-ECHS1 (IP:11305-1-AP, 3ug; Detection:11305-1-AP 1:500) with PC-3 cells lysate 3000ug.

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  IHC staining of human colon using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11305-1-AP (ECHS1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11305-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11305-1-AP (ECHS1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11305-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (11305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:600-1:2400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11305-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ECHS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ECHS1 fusion protein Ag1842
• Vollständiger Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 29-31 kDa
• GenBank-Zugangsnummer: BC008906
• Gene symbol: ECHS1
• Gene ID (NCBI): 1892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Publikationen

Species	Application	Title
human	IF	Drug Resist Updat; MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance; Authors - GuoSheng Wang
human	WB	Cancer Res; One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.; Authors - Yeung Anthony T AT
rat	WB	J Proteome Res; Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.; Authors - Cieniewski-Bernard Caroline C
human	WB	J Proteome Res; Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.; Authors - Gek San Tan
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	WB	Am J Pathol; The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.; Authors - Addabbo Francesco F