EDC3 Polyklonaler Antikörper

EDC3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 16486-1-AP

Synonyme

YJDC, PP844, LSM16 homolog, LSM16, hYjeF_N2

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  WB analysis using 16486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16486-1-AP

  IP result of anti-EDC3 (IP:16486-1-AP, 4ug; Detection:16486-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16486-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EDC3 antibody (16486-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

16486-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EDC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EDC3 fusion protein Ag9608
• Vollständiger Name: enhancer of mRNA decapping 3 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 508 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC021271
• Gene symbol: EDC3
• Gene ID (NCBI): 80153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
mouse	IF	PLoS Genet; Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association; Authors - Lauren G Chukrallah
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
human		Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak
human	IF,WB	J Cell Biol; DDX6 modulates P-body and stress granule assembly, composition, and docking; Authors - Nina Ripin
human	WB	EMBO Rep; Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6; Authors - Emanuela Senatore

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

great with ecl

• Applications: Immunohistochemistry