EEA1 Monoklonaler Antikörper
EEA1 Monoklonal Antikörper für IF, ELISA
Wirt / Isotyp
Maus / IgM
Getestete Reaktivität
human
Anwendung
WB, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1G8B2
Kat-Nr. : 66218-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 1 publications below |
| IF | See 2 publications below |
Produktinformation
66218-1-Ig bindet in WB, IF, ELISA EEA1 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human |
| Wirt / Isotyp | Maus / IgM |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | early endosome antigen 1 |
| Berechnetes Molekulargewicht | 162 kDa |
| GenBank-Zugangsnummer | NM_003566 |
| Gene symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Thiophiler Affinitätschromatograph |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| IF protocol for EEA1 antibody 66218-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Theranostics Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma. | ||
J Virol The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance of Stress Granules by Promoting Amphisome Formation. | ||
Eur J Pharmacol SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Azita (Verified Customer) (06-16-2021) | Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cell line that was pre-treated by the drug of interest and labelled by EEA1 monoclonal antibody at dilution of 1:50 showed strong labelling of early endosomes.
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