EEA1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

EEA1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C11

Kat-Nr. : 81245-1-RR

Synonyme

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

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  WB analysis of HepG2 using 81245-1-RR

  WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells.

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  WB analysis using 81245-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81245-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81245-1-RR bindet in WB, ELISA EEA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EEA1 fusion protein Ag28814
• Vollständiger Name: early endosome antigen 1
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: NM_003566
• Gene symbol: EEA1
• Gene ID (NCBI): 8411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.