EEF1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EEF1A1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 11402-1-AP

Synonyme

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, GRAF 1EF, HNGC:16303, LENG7, PTI1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11402-1-AP

  WB result of EEF1A1 antibody (11402-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HeLa cells.

• 

  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11402-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11402-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11402-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11402-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11402-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11402-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11402-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11402-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11402-1-AP

  Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C2C12-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, Maus-Eierstockgewebe, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Neuro-2a-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11402-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA EEF1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEF1A1 fusion protein Ag1938
• Vollständiger Name: eukaryotic translation elongation factor 1 alpha 1
• Berechnetes Molekulargewicht: 462 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC014224
• Gene symbol: EEF1A1
• Gene ID (NCBI): 1915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Publikationen

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Nat Commun; Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction; Authors - Minji Kim ; KD Validated
human,mouse	WB,IF	Nat Chem Biol; Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis; Authors - Yuyin Pan
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
human	WB,IF	Cancer Res; The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.; Authors - Xiuxia Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

• Applications: Western Blot
• Cell Tissue Type: Heart

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