EEF1A1/EEF1A2 Monoklonaler Antikörper

EEF1A1/EEF1A2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4F3F4

Kat-Nr. : 66806-1-Ig

Synonyme

EEF1A2, 4F3F4, eEF1A-2, EEF1AL, EF-1-alpha-2

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  WB analysis using 66806-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66806-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66806-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66806-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 66806-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 66806-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66806-1-Ig bindet in WB, IHC, ELISA EEF1A1/EEF1A2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1A1/EEF1A2 fusion protein Ag6875
• Vollständiger Name: eukaryotic translation elongation factor 1 alpha 2
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC000432
• Gene symbol: EEF1A2
• Gene ID (NCBI): 1917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).