EEF1B2 Monoklonaler Antikörper

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• KD/KO Validated

EEF1B2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3E1C11

Kat-Nr. : 60329-1-Ig

Synonyme

3E1C11, EEF1B, EEF1B1, EF 1 beta, EF1B

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  WB analysis using 60329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:2900 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60329-1-Ig

  WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

• 

  WB analysis using 60329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit brain using 60329-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60329-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60329-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 60329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60329-1-Ig (EEF1B2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 60329-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (60329-1-Ig, Clone: 3E1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60329-1-Ig bindet in WB, IHC, IF/ICC, ELISA EEF1B2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EEF1B2 fusion protein Ag0135
• Vollständiger Name: eukaryotic translation elongation factor 1 beta 2
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC000211
• Gene symbol: EEF1B2
• Gene ID (NCBI): 1933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.