EEF2 Polyklonaler Antikörper

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• KD/KO Validated

EEF2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 20107-1-AP

Synonyme

EEF 2, EEF2, EF 2, EF2, Elongation factor 2

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Galerie der Validierungsdaten

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  WB analysis using 20107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20107-1-AP

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells.

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  WB analysis of HeLa using 20107-1-AP

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 20107-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20107-1-AP

  IP Result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20107-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20107-1-AP

  Immunofluorescent analysis of HepG2 cells, using EEF2 antibody 20107-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 20107-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

20107-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA EEF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EEF2 fusion protein Ag13826
• Vollständiger Name: eukaryotic translation elongation factor 2
• Berechnetes Molekulargewicht: 858 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 100 kDa
• GenBank-Zugangsnummer: BC126259
• Gene symbol: EEF2
• Gene ID (NCBI): 1938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
zebrafish	WB	Nature; A molecular network of conserved factors keeps ribosomes dormant in the egg; Authors - Friederike Leesch
human	WB	Cancer Lett; eEF-2 Kinase-targeted miR-449b confers radiation sensitivity to cancer cells.; Authors - Cheng Ji
human	WB,CoIP,RIP	Carcinogenesis; PLAGL2 promotes Snail expression and gastric cancer progression via UCA1/miR-145-5p/YTHDF1 axis; Authors - Wen Chen
human	WB	Biomed Microdevices; Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.; Authors - Hao-Peng Lin
human	WB	Pathol Res Pract; miR-183-5p acts as a potential prognostic biomarker in gastric cancer and regulates cell functions by modulating EEF2.; Authors - Wang Li
human	WB	BMC Nephrol; Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.; Authors - Yang Lu

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C