Pikachurin Monoklonaler Antikörper

Pikachurin Monoklonal Antikörper für WB, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1C6D12

Rezensionen (1)

Kat-Nr. : 66232-1-Ig

Synonyme

EGFLAM, 1C6D12, Agrin like protein, AGRINL, Agrin-like protein

---

• 

  WB analysis of human retina using 66232-1-Ig

  human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66232-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of rat eye using 66232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Y79 using 66232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Retina-Gewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF-P: Ratten-Augengewebe
• Erfolgreiche Detektion in IF/ICC: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

66232-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA Pikachurin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Pikachurin fusion protein Ag6362
• Vollständiger Name: EGF-like, fibronectin type III and laminin G domains
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa, 55-60 kDa
• GenBank-Zugangsnummer: BC033177
• Gene symbol: Pikachurin
• Gene ID (NCBI): 133584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Publikationen

Species	Application	Title
human	IHC	Int Immunopharmacol; EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma; Authors - Kaishu Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Federico (Verified Customer) (02-18-2020)

The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 2000x
• Cell Tissue Type: overexpressing protein samples from HEK293 cells, conditioned media from Y79 cells