Pikachurin Monoklonaler Antikörper

Pikachurin Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6D12

Rezensionen (1)

Kat-Nr. : 66232-1-Ig

Synonyme

Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin

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Galerie der Validierungsdaten

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  WB analysis of human retina using 66232-1-Ig

  human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66232-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Y79 using 66232-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Retina-Gewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66232-1-Ig bindet in WB, IF, ELISA Pikachurin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Pikachurin fusion protein Ag6362
• Vollständiger Name: EGF-like, fibronectin type III and laminin G domains
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa, 55-60 kDa
• GenBank-Zugangsnummer: BC033177
• Gene symbol: EGFLAM
• Gene ID (NCBI): 133584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federico (Verified Customer) (02-18-2020)

The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific.

• Applications: Western Blot,
• Primary Antibody Dilution: 2000x
• Cell Tissue Type: overexpressing protein samples from HEK293 cells, conditioned media from Y79 cells