EGFR Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EGFR Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

51

CloneNo.

2A2H10

Rezensionen (4)

Kat-Nr. : 66455-1-Ig

Synonyme

EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Galerie der Validierungsdaten

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  WB analysis using 66455-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66455-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66455-1-Ig

  WB result of EGFR antibody (66455-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR transfected HepG2 cells.

• 

  WB analysis of EC109 using 66455-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66455-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66455-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66455-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66455-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66455-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66455-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 66455-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66455-1-Ig (EGFR antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66455-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66455-1-Ig (EGFR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of A431 using 66455-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human EGFR (66455-1-Ig, Clone:2A2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, EC109-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, MDA-MB-231-Zellen, MDA-MB-468-Zellen, PC-3-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Gliomgewebe, humanes Hautkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 45 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Produktinformation

66455-1-Ig bindet in WB, IHC, IF, FC, ELISA EGFR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EGFR fusion protein Ag24947
• Vollständiger Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Berechnetes Molekulargewicht: 1210 aa, 134 kDa
• Beobachtetes Molekulargewicht: 145-165 kDa
• GenBank-Zugangsnummer: BC094761
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Publikationen

Species	Application	Title
human	WB	Mol Cell; N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.; Authors - Zihao Dai ; KD Validated
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu
human	WB,IHC	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
human	IHC	Cell Death Dis; Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR.; Authors - Xiao-Wei Zhang
human	WB	Phytomedicine; Maintenance of airway epithelial barrier integrity via the inhibition of AHR/EGFR activation ameliorates chronic obstructive pulmonary disease using effective-component combination; Authors - Yanxin Wei
human	WB,IF	J Transl Med; Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1; Authors - Ruijiao Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k. (Verified Customer) (10-26-2023)

This antibody worked well for human cells and mouse liver cell proteins at 1:500 or 1:1000 concentrations at 4 °C over a night of incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

FH

Christos (Verified Customer) (02-13-2023)

-35ug protein extract were loaded per well -Transfer was performed for 2hr at 400mA at 4oC, on a Nitrocellulose Blotting Membrane -Membrane blocking was performed in 5% non-fat milk in PBS-Tween20 at room temperature, under mild shacking -Antibodies dilutions were performed in 5% non-fat milk in PBS-Tween20. -Incubations with the primary antibodies were performed as followed: 1)EGFR: 1:1000 for 1.5hr at 4oC 2)tubulin (sc32293, Santa Cruz): 1:5000 for 1.5hr at 4oC -Incubations with the secondary antibodies were performed with Rb pAb to Ms IgG (HRP) (ab 6728, Abcam) at a 1:20000 dilution, for 1hr at 4oC.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human breast cancer cells/ SKBR3, MDA-MB-231

EGFR Antibody Western Blot validation (1/1000 dilution) in Human breast cancer cells/ SKBR3, MDA-MB-231 (Cat no:66455-1-Ig)

FH

Guorong (Verified Customer) (03-31-2022)

A band of approximately 160 kDa was detected

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

EGFR Antibody Western Blot validation (1:1000 dilution) in ARPE-19 (Cat no:66455-1-Ig)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other