EIF2A/CDA02 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF2A/CDA02 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G7H4

Rezensionen (1)

Kat-Nr. : 66482-1-Ig

Synonyme

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 66482-1-Ig

  WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells.

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  WB analysis using 66482-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of ROS1728 using 66482-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66482-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66482-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66482-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66482-1-Ig (EIF2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66482-1-Ig bindet in WB, IHC, IF, ELISA EIF2A/CDA02 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EIF2A/CDA02 fusion protein Ag22041
• Vollständiger Name: eukaryotic translation initiation factor 2A, 65kDa
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC011885
• Gene symbol: EIF2A
• Gene ID (NCBI): 83939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

Publikationen

Species	Application	Title
human	WB	Pharm Biol; Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway; Authors - Yaoyao Bian
human	WB	Front Neurosci; Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease; Authors - Fahad Mubariz

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Dorota (Verified Customer) (04-10-2019)

Very good for western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250 -1:1000
• Cell Tissue Type: U2OS

EIF2A/CDA02 Antibody Western Blot, validation (1:250 -1:1000 dilution) in U2OS (Cat no:66482-1-Ig)