EIF2S3 Polyklonaler Antikörper

EIF2S3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11162-1-AP

Synonyme

eIF 2 gamma X, eIF 2gA, eIF 2gX, EIF2, EIF2G, EIF2gamma, EIF2S3

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11162-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11162-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11162-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11162-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11162-1-AP

  IP Result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug.

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11162-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11162-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

11162-1-AP bindet in WB, IP, IHC, IF, ELISA EIF2S3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF2S3 fusion protein Ag1650
• Vollständiger Name: eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC019906
• Gene symbol: EIF2S3
• Gene ID (NCBI): 1968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]

Publikationen

Species	Application	Title
human	WB	Nat Neurosci; Deletion of TOP3β, a component of FMRP-containing mRNPs, contributes to neurodevelopmental disorders.; Authors - Stoll Georg G
human	WB	Mol Biol Cell; PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation.; Authors - Nasim Haghandish