eIF4G2/DAP5 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

eIF4G2/DAP5 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

3C11A3

Kat-Nr. : 67428-1-Ig

Synonyme

EIF4G2, 3C11A3, AAG1, DAP 5, DAP5

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  WB analysis using 67428-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (EIF4G2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67428-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67428-1-Ig (eIF4G2/DAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67428-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67428-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67428-1-Ig (EIF4G2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67428-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eIF4G2/DAP5 antibody (67428-1-Ig, Clone: 3C11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

67428-1-Ig bindet in WB, IHC, IF/ICC, ELISA eIF4G2/DAP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: eIF4G2/DAP5 fusion protein Ag12080
• Vollständiger Name: eukaryotic translation initiation factor 4 gamma, 2
• Berechnetes Molekulargewicht: 907 aa, 102 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC043149
• Gene symbol: EIF4G2
• Gene ID (NCBI): 1982
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Cell; ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.; Authors - Yichen Xu ; KD Validated
human	WB	Autophagy; CircRNA GRAMD4 induces NBR1 expression to promote autophagy and immune escape in renal cell carcinoma; Authors - Mi Zhou