ELOF1 Polyklonaler Antikörper

ELOF1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21070-1-AP

Synonyme

ELF1

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  WB analysis using 21070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 21070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 21070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ELOF1 antibody (21070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of SKOV-3 using 21070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using ELOF1 antibody (21070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21070-1-AP bindet in WB, IF/ICC, ELISA ELOF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ELOF1 fusion protein Ag15213
• Vollständiger Name: elongation factor 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 9.5 kDa
• Beobachtetes Molekulargewicht: 9 kDa
• GenBank-Zugangsnummer: BC007516
• Gene symbol: ELOF1
• Gene ID (NCBI): 84337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ELOF1 (elongation factor 1 homologue) is a small (83 aa), evolutionarily conserved, RNAPII-associated transcription elongation factor that is also a core component of the transcription-coupled DNA repair machinery with an additional role in preventing DNA damage during DNA replication (PMID: 34108663). Loss of ELOF1 strongly compromises AID-mediated deamination, SHM, and CSR, and that this is accompanied by a decrease in parameters of RNAPII pausing/stalling and in RNAPII RNA synthesis without a decrease in levels of S2P-RNAPII (PMID: 39386505).