EMILIN1 Monoklonaler Antikörper

EMILIN1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A5C8

Kat-Nr. : CL488-60047

Synonyme

DKFZp586M121, EMI, EMILIN, EMILIN 1, EMILIN1, gp115

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Galerie der Validierungsdaten

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  IF Staining of human ovary tumor using CL488-60047

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

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  IF Staining of human ovary tumor using CL488-60047

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Ovarialkarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60047 bindet in IF EMILIN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EMILIN1 fusion protein Ag1016
• Vollständiger Name: elastin microfibril interfacer 1
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC007530
• Gene symbol: EMILIN1
• Gene ID (NCBI): 11117
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).